Continuously adjustable pipette operation and calibration procedures
2. Scope of application: Continuously adjustable pipettes of various brands and models.
3. Operating procedures
3.1 Turn the knob to set the amount of pipetting. The set pipetting amount should not exceed the range specified by the pipette.
3.2 Install the matching tip (Tip).
3.3 Press the button to the first stop position.
3.4 Dip the pipette tip 2 to 3 mm deep below the liquid surface, then slowly release the button to draw in the liquid. After moving the tip into the liquid, remove the tip from the liquid surface and wipe off any droplets on the outside of the tip.
3.5 Gently press the operation button to the first stop position to release the liquid. After about 1 second, continue to press the operation button down to the second stop position. After the liquid to be sucked is cleaned, the tip is attached to the wall of the container to prevent droplets from dripping into the bottle, and the tip is withdrawn.
3.6 Release the button to return it to the starting position. The tip can be replaced as needed to continue the pipetting operation.
4. Calibration method
4.1. Calibration environment and appliance requirements Room temperature: 20 ~ 25 ° C, the fluctuation range in the measurement is not more than ± 0.5 ° C.
Electronic balance: placed on a countertop that is free of dust and vibration, and the room is as air-conditioned as possible. When weighing, in order to ensure the humidity in the balance (relative humidity 60 ~ 90%), a small beaker containing 10mL of distilled water should be placed in the balance.
Small beaker: 5 to 10 mL volume.
Determination of liquid: degassed double distilled water at a temperature of 20 to 25 °C.
Selected calibration volume:
a) the volume to be calibrated;
b) The intermediate volume of the pipette calibration volume.
c) Minimum adjustable volume (not less than 10% of the intended volume).
In the case of a fixed volume pipette, there is only one calibration volume.
4.2. Calibration procedure
a) adjust the pipette to the volume to be calibrated and select the appropriate tip;
b) adjust the balance;
c) sucking distilled water back and forth 3 times to make the tip wet, and dry the tip with gauze;
d) Hold the pipette vertically, immerse the tip in the liquid surface 2 to 3 mm, and slowly absorb the distilled water (1 to 3 seconds);
e) remove the tip from the liquid surface and lean against the wall to remove the liquid from the outside of the tip;
f) Place the pipette into the weighing beaker at an angle of 30 ° C, slowly and consistently press the pipette to the first gear, wait 1 to 3 seconds, then press to the second gear to make the liquid in the nozzle completely discharge;
g) record the weighing value;
h) wipe the outside of the tip;
i) Weigh 10 times according to the above steps;
j) taking the average of 10 measurements as the weight of distilled water taken by the last pipette, and calculating the volume according to the Z factor of distilled water listed in Schedule 1;
k) Adjust the pipette according to the calibration result.
Our Laser module is make up with high precision laser diode and APC, ACC drive circuit and coating optical glass lenses with high performance. With high reliability, stability, strong anti-interference, good consistency, long service life and so on.
Worldhawk offers a range of lasers including a broad selection of Laser Diode Modules in colors such as red, green, blue, or violet.
These laser module can be used in Industrial marking devices, laser medical equipment, stage lighting and laser targeting devices, Machine vision, Laser Alignment, Laser Positioning Surveying Instrument, Laser Sight and so on.
Wavelength(color):
-Blue-violet (blue): 405nm, 450nm
-Green: 520nm, 532nm
-Red: 635nm, 650nm, 658nm, 660nm, 670nm,685nm
-IR(infrared): 780nm, 808nm, 830nm, 850nm, 905nm, 940nm, 980nm, 1064nm
Beam model: Cross line, Straight line, Dot(Point)
Output Power: 1mW ~ 200mW
Certification: CE/ FDA/ Rohs.
Laser Module,Dot Laser Module,Green Laser Module,Laser Diode Module
ChangChun Worldhawk Optics Co.,Ltd , https://www.worldhawk-optics.com