Formulation of Agaricus bisporus three-grade strains

Most of the third-class species of Agaricus bisporus are wheat grains. However, in actual production, the pollution rate is high, or abnormal phenomena occur, which makes it difficult to implement the production plan. The reason is that the production operation of the strains is not standardized. Now, to the production time of the third-class species of Agaricus bisporus, the specific specification and operating techniques are specifically proposed for reference by the farmer friends.

1. Raw material treatment: Cow manure is sifted and sifted, mixed with 1:1.2 water, mix and ferment, and piled once a day. About 20 days, cow's manure powder turns into brown, with the normal scent of fermented forage, drying Dry standby. Without moisture, wheat is soaked thoroughly and then removed. It is shaded and ventilated properly. When the epidermis is dry, it is lightly sprayed with fine mist and moistens. It can germinate in 24 hours and can be used when the malt grows to 1 to 2 centimeters.

If you have worm-eaten wheat grains, you can also use them, but the grains behind the insects have holes and the starch is easy to flow out. Therefore, you must wash them several times; then pour in lime water with a PH value of 8-9, and soak for 6 hours. About 20 minutes, until the wheat kernels have no white heart, remove the grains and rinse them with cold water.

2. Basic formula: 100 kg of wheat (dry goods), 30 kg of cow dung powder, 1 kg of gypsum powder, and 1.5 kg of calcium carbonate.

3. Substrate mixing: Mix wheat kernels with cow dung powder in a ratio of 1:0.2, and mix in other auxiliary materials at the same time. Make sure that cow dung is completely adhered to the wheat kernels. The remaining cow dung powder is ready for use.

4. Bottling operation: In general, small batch production does not use standard bottles, but uses bottles, glucose glass bottles, and bottle sterilization. To reduce production costs, 500 ml infusion bottles are available. First put a cow dung powder at the bottom of the bottle, about 1~1.5 cm thick, and then put the grain of wheat. When the bottle is bottled, make the grain 1 cm below the shoulder of the bottle. Then, load 1~2 bottles. Cow dung powder, and use "L"-shaped tools to flatten it, crushed, and then stuffed tampon.

5. Sterilization cooling: Using autoclave method, the pressure is maintained at 0.15 MPa for 2 to 2.5 hours. After sterilization, after the pressure has dropped to zero, the material bottle can be taken out, moved into the cooling room or inoculation room, cleaned up within 3 days in advance, and sprayed with silly solution; until the bottle is cooled to 30°C, it can be inoculated.

6. Inoculation operation: After indoor spraying once again with Saibai 09 solution, the original species will be scrubbed once with 75% alcohol to open the inoculation purifier, and the inoculation operation will be started after 10 minutes. Each bottle (500 ml) can receive more than 40 bottles.

7. Bacterial culture: The culture room (shed) is cleaned in advance, the ground is filled with water, and carpet-type spraying silly solution is applied. After 2 days of confinement, lime powder is applied on the ground to transfer the inoculated strains. During germination, try to control the temperature at 25 °C, not more than 28 °C, the installation of water temperature air conditioning can be reduced to about 22 °C, general farmers do not have the conditions, can be used to cool the soil: the fungus shed floor code brick 20 cm High, put a new plastic film, the strainer bottles into the vertical type, poured into the groundwater, changing the water 1 to 2 times a day, you can keep the temperature inside the bacteria bottle below 25 °C. Spray the drug every 3 days or so for prevention, and use silly and Saibai 09 alternately; when adults with mushrooms and mosquitoes are found, spray with beta cypermethrin immediately.

8. Miscellaneous treatment: On the third day after inoculation, check the miscellaneous items and find that any non-Agaricus mycelium bacteria bottle was immediately removed from the outside and was contaminated with Streptomyces spp., and the convenient bottle was used to cap the bacteria bottle from the top down. Slowly removed. For the contamination of general bacteria, the material can be poured out after spraying the drug, dried and reused, and the material should be removed from the culturing chamber and burned or buried deeply. The empty bottle should be heated above 70°C. Water scald and wash it before use.

9. Temporary storage: After 35 to 45 days, bacteria strains are ready to be sterilized. The strains that are not used temporarily should be stored at a low temperature, generally maintaining 2°C to 4°C.

10. Quality inspection: Agaricus bisporus mycelium is thick and clear, strong growth, fan-shaped growth, villous mycelium, neat, aerial hyphae white; now the most used "2796" is a semi-aerobic type, its The mycelium is prosperous and uniform; there are no thick yellow-white bacteria; the mycelium is thick and thick, and it is yellowish when exposed to high temperatures, and a small amount of bunchy mycelium can appear, which is uniform from top to bottom.

The growth of mycelium in the bottle is not robust, it is in a scattered state, or there is a phenomenon of “fungus elimination” and “bacterial breakage”. The material turns color, agglomerates or shrinks, and white, light yellow round blocks or irregular dark spots appear. , Miscellaneous bacteria and mite breeding, no mushroom flavor, with bleach or stench, etc., are unqualified bacteria. If the mycelium is light yellowish white, and the hyphae are atrophied, the growth is weak, or bacteria are formed during growth, the culture medium is a wet or older strain; the mycelium in the upper part of the strainer bottle shrinks and dries, and the lower part Hyphae growth is still good, the medium is too dry, the culture temperature is too high; if the growth is not uniform, it indicates that the wheat soaking and precooking time is not enough; if the growing mycelium is yellow, it means that the culture temperature is too high; if the bacteria species within the bottle There is a very thick quilt at the top, which is a species with poor performance or physiological characteristics. It should not be used. The strains that have been cultured under high temperature conditions are not easy to eat after sowing and have slow bacterial growth.

Removable Oxygen Cylinder Filling System

Compressed air is purified through the air dryer and filters to a certain level for main plant to work with. Air buffer is incorporated for smooth supply of compressed air thus to reduce fluctuation of compressed air source. The plant produces oxygen with PSA (pressure swing adsorption) technology, which is a time proven oxygen generation method. Oxygen of desired purity at 93%±3% is delivered to oxygen buffer tank for smooth supply of product gas. Oxygen in buffer tank is maintained at 4bar pressure and fill in cylinder with an oxygen booster and compress the oxygen purity to 150bar. 

With a customized container, all the parts can be involved in, and removable with a vehicle. 

Removable Oxygen Cylinder Filling System

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