Separation of pulp tissue
Separation of pulp tissue
Reagents and materials:
1. PBS balanced salt solution (PBSA) without magnesium and calcium;
2. MSC medium: α-MEM containing 2mmol/L glutamine, adding 15% fetal bovine serum, 0.1mmol/L L-ascorbate phosphate, 100U/ml penicillin and 100μg/ml streptomycin;
3. Petri dishes;
4. Fine é•Š (small and large);
5. Dental carbonized drill bits;
6. Tooth digger;
7. High speed dental handpieces;
8. Equipment when using a high-speed handpiece (such as an air compressor);
9. Disinfectant (eg povidone iodine);
experimental method:
1. Wash three times with PBSA to thoroughly clean the surface of the teeth;
2. Disinfect with disinfectant and then rinse thoroughly with PBSA;
3. Using a dental carbonized drill bit attached to a dental handpiece, cut the teeth along the junction of bone and enamel and expose the medullary cavity. The teeth were intermittently soaked in PBSA pre-cooled with ice to avoid overheating during the cutting process. In the case of SHED separation, sometimes this step is not required because the root absorbing pulp has been exposed;
4. Use a small fine sputum or tooth excavator to gently separate the pulp tissue from the pulp cavity;
5. Place the pulp tissue into a small amount of MSC medium in a Petri dish. Be careful not to dry the tissue;
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