Determination of rutin content in three kinds of loquat leaves
2019-09-13 06:06:02
Abstract: Objective: To provide reference for the development and utilization of natural resources of loquat leaves. Methods: The content of rutin in three kinds of loquat leaves was determined by ultraviolet spectrophotometry . The wavelength of 510nm was used, the correlation coefficient was r=0.9996, and the regression equation was C=0.073A+0000. RESULTS: Three kinds of loquat leaves of Chinese wolfberry, black locust and dragon clawed pheasant contained a certain amount of rutin, 2.294% of the national carp, 3.329% of the locust, and 2.084% of the dragon scorpion. Conclusion: The three kinds of loquat leaves have high rutin content in the hedgehog, which has certain development value.
Key words: ultraviolet spectrophotometry; loquat leaf; rutin; content determination Rutin is mainly extracted from the flower buds of the leguminous plant (Sephora japonica L.). In recent years, with the widening of the medical application of rutin, flower bud extraction has been far from meeting the market demand. There are abundant hedgehog resources all over the country, and dragon claws are also partially planted. In order to develop a new source of rutin, this experiment was carried out, three kinds of eucalyptus leaves of Sephora japonica L., Robinia pseudoacacia L, Sophora iaponica L.f.pendula Loud.Arb.Brit Determination of the content of rutin. Ultraviolet spectrophotometry was used. In order to broaden the natural source of rutin and the development and utilization of eucalyptus leaves to provide a reference.
1 instruments and reagents
S54 ultraviolet spectrophotometer (Shanghai prism), ultrasonic oscillation extractor (Kunshan, KQ-500E); rutin reference product provided by China National Institute for the Control of Pharmaceutical and Biological Products (for content determination), batch number: 0080-9705. The reagents used were of analytical grade (AR). The three kinds of eucalyptus leaves were collected from the courtyard of Taishan Medical College. The picking time was May 16, 2003, and it was naturally dry.
2 Methods and results
2.1 Preparation of the reference solution.
Weigh accurately 10mg of rutin reference substance dried to constant weight at 105 ° C, placed in a 100ml volumetric flask, dissolved in 50ml of methanol, dilute to the mark with distilled water, shake well, that is. The concentration was 0.2 mg/ml.
2.2 Linear relationship investigation Precision draw reference solution 0,1,2,3,4,5,6ml, placed in a 25ml volumetric flask, add distilled water to 6ml, add 5% sodium nitrite 1ml, shake, rest 6 minutes, add 10% aluminum nitrate 1ml, shake, let stand for 6 minutes, add 4.3% sodium hydroxide 10ml, add distilled water to the mark, let stand for 15 minutes, absorb the absorbance with UV spectrophotometer, detection wavelength 510nm. Taking the absorption as the ordinate and the concentration as the abscissa, the computer shows that the regression equation is: C=0.073A+0.000, and the correlation coefficient is r=0.9996. It indicates that the concentration has a good linear relationship in the range of 0.008 to 0.048 mg/ml.
2.3 Determination of the sample Weigh 10g of dried eucalyptus leaves, crushed 40 mesh sieve, add 100ml of hot water about 50 °C, a little boron borax, adjust the pH value of calcium hydroxide aqueous solution 8 ~ 9, super-oscillation extraction 2 Times, each time 10 minutes, the ultrasonic frequency is 500 W, the extraction temperature is 60 ° C, the filtrate is combined twice, the concentrated sulfuric acid is added to adjust the pH value of 3 to 4, and it is left for 4 hours, and it is refrigerated overnight in a refrigerator. After suction filtration, the mixture was washed with water to neutrality, and the precipitate was transferred to a beaker. 100 ml of anhydrous methanol was added, and heated in a water bath. After being dissolved, a small amount of activated carbon was added, heating was continued for 10 minutes, filtered while hot, and the filtrate was allowed to cool to room temperature. 10 ml of the precise amount of sample solution, placed in a 100 ml volumetric flask, add water to the mark and shake. Accurately absorb the 3m1 25m1 volumetric flask, measure the absorbance according to the method of 2.2, and calculate the rutin content of the three loquat leaves according to the dry product.
2.4 Stability test Take sample No. 2, measure the absorbance according to item 2.2, measure once every 15 minutes, calculate the mean and RSD, the results show that the sample solution is stable within 75 minutes.
2.5 Sample recovery test Take 5 samples of the sample No. 2 sample, and add rutin reference substance, measure according to the method of 2.2, calculate the recovery rate and RSD.
3 Discussion
3.1 At present, the methods for determining the content of rutin in plants mainly include high performance liquid chromatography and ultraviolet spectrophotometry. This experiment was designed using a high-performance liquid phase method, but the viscosity of the extract is large and it is difficult to process into a sample liquid that meets the conditions for high-performance liquid chromatography. The method of determining the content of rutin in glutinous rice by UV spectrophotometry of the 2000 edition of the Pharmacopoeia of the People's Republic of China was carried out. And the decoction method and the Soxhlet extraction method are ultrasonic extraction methods. Through the determination of rutin content in three kinds of loquat leaves, the system stability, precision and recovery rate of the method were proved to be satisfactory. UV spectrophotometry can be used to determine the content of rutin in loquat leaves.
3.2 The type and content of chemical components in different parts of the same original plant source, and the harvesting time of the same part are different in the same component. The results of this experiment indicated that the three kinds of loquat leaves of the Chinese wolfberry, black locust and dragon clawed pheasant contained higher levels of rutin, which was higher than that reported in the literature. Among them, the rutin content of the locust leaves was high.
3.3 Through this experiment, to provide a reference for further expansion of the natural source of rutin, at the same time, to lay a certain foundation for the further development of eucalyptus preparation.
references:
[1] Pharmacopoeia Commission of the People's Republic of China. (Pharmacopoeia of the People's Republic of China) [M]. One. 2000.
[2] Qian Yancong, Liu Jingdong, Yan Wenhui, et al. Determination of rutin content in the leaves of P. chinensis by TLC scanning [J]. Basic Chinese Medicine Journal, 2002, 16 (1): 26-27.
Key words: ultraviolet spectrophotometry; loquat leaf; rutin; content determination Rutin is mainly extracted from the flower buds of the leguminous plant (Sephora japonica L.). In recent years, with the widening of the medical application of rutin, flower bud extraction has been far from meeting the market demand. There are abundant hedgehog resources all over the country, and dragon claws are also partially planted. In order to develop a new source of rutin, this experiment was carried out, three kinds of eucalyptus leaves of Sephora japonica L., Robinia pseudoacacia L, Sophora iaponica L.f.pendula Loud.Arb.Brit Determination of the content of rutin. Ultraviolet spectrophotometry was used. In order to broaden the natural source of rutin and the development and utilization of eucalyptus leaves to provide a reference.
1 instruments and reagents
S54 ultraviolet spectrophotometer (Shanghai prism), ultrasonic oscillation extractor (Kunshan, KQ-500E); rutin reference product provided by China National Institute for the Control of Pharmaceutical and Biological Products (for content determination), batch number: 0080-9705. The reagents used were of analytical grade (AR). The three kinds of eucalyptus leaves were collected from the courtyard of Taishan Medical College. The picking time was May 16, 2003, and it was naturally dry.
2 Methods and results
2.1 Preparation of the reference solution.
Weigh accurately 10mg of rutin reference substance dried to constant weight at 105 ° C, placed in a 100ml volumetric flask, dissolved in 50ml of methanol, dilute to the mark with distilled water, shake well, that is. The concentration was 0.2 mg/ml.
2.2 Linear relationship investigation Precision draw reference solution 0,1,2,3,4,5,6ml, placed in a 25ml volumetric flask, add distilled water to 6ml, add 5% sodium nitrite 1ml, shake, rest 6 minutes, add 10% aluminum nitrate 1ml, shake, let stand for 6 minutes, add 4.3% sodium hydroxide 10ml, add distilled water to the mark, let stand for 15 minutes, absorb the absorbance with UV spectrophotometer, detection wavelength 510nm. Taking the absorption as the ordinate and the concentration as the abscissa, the computer shows that the regression equation is: C=0.073A+0.000, and the correlation coefficient is r=0.9996. It indicates that the concentration has a good linear relationship in the range of 0.008 to 0.048 mg/ml.
2.3 Determination of the sample Weigh 10g of dried eucalyptus leaves, crushed 40 mesh sieve, add 100ml of hot water about 50 °C, a little boron borax, adjust the pH value of calcium hydroxide aqueous solution 8 ~ 9, super-oscillation extraction 2 Times, each time 10 minutes, the ultrasonic frequency is 500 W, the extraction temperature is 60 ° C, the filtrate is combined twice, the concentrated sulfuric acid is added to adjust the pH value of 3 to 4, and it is left for 4 hours, and it is refrigerated overnight in a refrigerator. After suction filtration, the mixture was washed with water to neutrality, and the precipitate was transferred to a beaker. 100 ml of anhydrous methanol was added, and heated in a water bath. After being dissolved, a small amount of activated carbon was added, heating was continued for 10 minutes, filtered while hot, and the filtrate was allowed to cool to room temperature. 10 ml of the precise amount of sample solution, placed in a 100 ml volumetric flask, add water to the mark and shake. Accurately absorb the 3m1 25m1 volumetric flask, measure the absorbance according to the method of 2.2, and calculate the rutin content of the three loquat leaves according to the dry product.
2.4 Stability test Take sample No. 2, measure the absorbance according to item 2.2, measure once every 15 minutes, calculate the mean and RSD, the results show that the sample solution is stable within 75 minutes.
2.5 Sample recovery test Take 5 samples of the sample No. 2 sample, and add rutin reference substance, measure according to the method of 2.2, calculate the recovery rate and RSD.
3 Discussion
3.1 At present, the methods for determining the content of rutin in plants mainly include high performance liquid chromatography and ultraviolet spectrophotometry. This experiment was designed using a high-performance liquid phase method, but the viscosity of the extract is large and it is difficult to process into a sample liquid that meets the conditions for high-performance liquid chromatography. The method of determining the content of rutin in glutinous rice by UV spectrophotometry of the 2000 edition of the Pharmacopoeia of the People's Republic of China was carried out. And the decoction method and the Soxhlet extraction method are ultrasonic extraction methods. Through the determination of rutin content in three kinds of loquat leaves, the system stability, precision and recovery rate of the method were proved to be satisfactory. UV spectrophotometry can be used to determine the content of rutin in loquat leaves.
3.2 The type and content of chemical components in different parts of the same original plant source, and the harvesting time of the same part are different in the same component. The results of this experiment indicated that the three kinds of loquat leaves of the Chinese wolfberry, black locust and dragon clawed pheasant contained higher levels of rutin, which was higher than that reported in the literature. Among them, the rutin content of the locust leaves was high.
3.3 Through this experiment, to provide a reference for further expansion of the natural source of rutin, at the same time, to lay a certain foundation for the further development of eucalyptus preparation.
references:
[1] Pharmacopoeia Commission of the People's Republic of China. (Pharmacopoeia of the People's Republic of China) [M]. One. 2000.
[2] Qian Yancong, Liu Jingdong, Yan Wenhui, et al. Determination of rutin content in the leaves of P. chinensis by TLC scanning [J]. Basic Chinese Medicine Journal, 2002, 16 (1): 26-27.
Such as Vomit bag, Ear Syringe, Three ball spirometer, Biohazard Specimen Bag, Hot Cold Pack, Ice bag and so on.
NINGBO YINGMED MEDICAL INSTRUMENTS CO.,LTD , https://www.chinayingmed.com