Video Time Difference Microscopy "Peeping" the Development of Human Embryos

Release date: 2010-11-16

Researchers report in the journal Nature-Biotechnology, which was published online a few days ago, that human embryos can be clearly seen through video time-lapse microscopy. This new achievement is expected to help regenerate clinical medicine to improve the successful implantation rate of in vitro fertilized embryos by selectively implanting embryos into the female uterus.
Renee Reijo Pera collaborated with colleagues to document visual cues that allowed them to predict whether a 2-day-old embryo could develop into a 5-day or 6-day-old embryo by recording the number of in vitro fertilized embryos after fertilization, at a structural biologist. There, this clue is called a blastocyst. They found that embryos with a high probability of developing into the blastocyst stage should meet three key conditions: the first is cytokinesis, the stage of cell division lasts for about 0 to 33 minutes; the mitosis in the first and second stages There is an interval of 7.8 to 14.3 hours, and there is a 0 to 5.8 hour interval between the second and third stages of mitosis. They also found that individual cells in a single embryo have different gene expression patterns, some contain more transcripts from the mother, and others have active embryonic gene expression.
If this method is applied to clinical in vitro fertilization embryo implantation projects, this non-invasive imaging method increases the success rate of embryo implantation, thereby increasing the conception rate.

Source: Science Times

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